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  2. qScript One-Step Fast RT Optimized 20X formulation of recombinant MMLV reverse transcriptase for one-step Fast qRT-PCR. One-Step Fast Master Mix, Low ROX (4X) 4X reaction buffer containing dNTPs, magnesium chloride, AccuStart Taq DNA polymerase, ROX Reference Dye (for 580-585 nm excitation), and stabilizers Nuclease-free water

  3. 1. Aug. 2021 · Consequently, a one-step fast and label-free imaging array was reported for simultaneously identifying and detecting H1N1, H7N9 and H5N1 within only 20 min and in a small volume solution (50 μL). Based on the grey-level analysis, the imaging array achieved the limit of detection of 136 pM, 141 pM and 129 pM, respectively. The on-site imaging array also displayed good mismatch discrimination ...

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  7. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. The One-Step Fast Master Mix is provided as a 4X ...