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2. link.springer.com › chapter › 10Gene Editing Tools for Engineering Beneficial Microorganism in...

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   Vor 6 Stunden · In the meantime, in rat and mice embryos, an additional manufactured I-CreI that was intended to disrupt a 22-bp stretch of the Rag1 gene showed successful targeting events (Ménoret et al. 2013). Consequently, to create a unique MegN, per new genetic engineering target needs to go through a preliminary protein design step. Therefore, even with the large number of MegNs that are currently ...