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In biochemistry, a Hanes–Woolf plot, Hanes plot, or plot of against is a graphical representation of enzyme kinetics in which the ratio of the initial substrate concentration to the reaction velocity is plotted against . It is based on the rearrangement of the Michaelis–Menten equation shown below:
[] = [] +; (Hanes-Woolf) Die Steigung dieser linearen Funktion beträgt 1 v m a x {\displaystyle \textstyle {\frac {1}{v_{\mathrm {max} }}}} ; sie schneidet die [ S ] v {\displaystyle \textstyle {\frac {[S]}{v}}} -Achse bei [ S ] v = K m v m a x {\displaystyle \textstyle {\frac {[S]}{v}}={\frac {K_{\mathrm {m} }}{v_{\mathrm {max ...
Although it is still used for representation of kinetic data, non-linear regression or alternative linear forms of the Michaelis–Menten equation such as the Hanes-Woolf plot or Eadie–Hofstee plot are generally used for the calculation of parameters.
Just like the Lineweaver-Burk equation, the Hanes-Woolf equation is of the form y = mx + c. The Hanes-Woolf plot is thought to be more accurate than Lineweaver-Burk for the determination of kinetic parameters. The plot and the equation are shown below.
The Hanes-Woolf plot has the form S ν = Km V m + 1 V mS S ν = K m V m + 1 V m S Therefore S/ν S / ν is plotted against S S and 1/V m 1 / V m is the slope an Km/V m K m / V m is the y-intercept.
11. Feb. 2019 · The Hanes-Woolf method shows effects of both error_1 and error_2 on precision; for values of error_2 exceeding 0.0020 mmol L −1 also a substantial fraction of negative results is obtained. Eadie-Hofstee results resemble the observations made for the implicit solution method: for smaller values of error_2 the precision is not too ...
5. Dez. 2017 · The equation describes the dependence of enzyme-catalyzed reaction rates on the concentration of substrate by using two parameters, the catalytic constant, k cat and the Michaelis-Menten...
