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Vor 5 Tagen · Western Blot Analysis is the most widely used technique for protein detection. In this video, we will cover the 3 major steps in generating a western blot: Separate, Transfer, and Detect.
- 3 Min.
Vor 6 Tagen · Western blotting, otherwise known as immunoblotting, is a common molecular biology technique that scientists use to detect and analyze proteins in a biological sample. 1 Total proteins from the sample are separated by size via gel electrophoresis, then transferred onto a nitrocellulose membrane, where specific target proteins are labeled using a...
Vor 2 Tagen · Learn how to perform a western blot transfer using the Mini Blot module in a Mini Gel Tank.
Vor einem Tag · Western Blot (Western Blot) is a commonly used technique for qualitative and quantitative detection of protein expression. The main purpose of Western Blot is to detect whether a certain protein (i.e. the target protein) is expressed in the sample (tissue or cells) and the relative abundance of expression. Specific antibodies identify and bind target proteins in cells or biological tissue ...
21. Juni 2024 · Learn how to transfer proteins from SDS-PAGE gels to membranes using the Invitrogen iBlot 2 Dry Blotting System.
20. Juni 2024 · Learn about our range of chemiluminescent western blot detection reagents including HRP substrates and AP substrates and the advantages of both.
24. Juni 2024 · Harsha Rao (HR): Stain-Free western blotting is a method that enables total protein normalization without the need for traditional staining and destaining. With Stain-Free western blotting, protein separation is completed by electrophoresis and the gel is activated under UV light.
