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Vor 5 Tagen · Western Blotting is the most widely used technique for protein detection. In this video, we will cover the 3 major steps in generating a western blot: Separate, Transfer, and Detect. After protein samples have been prepared, the mixture is separated by molecular weight using protein gel electrophoresis, which is a standard laboratory ...
- 3 Min.
Vor 4 Tagen · In this video, you’ll learn how to transfer proteins from polyacrylamide gels to membranes using the Invitrogen iBlot2 Dry Blotting System. The iBlot2 Dry Blotting System uses pre-assembled transfer stacks with integrated nitrocellulose or PVDF membranes, offering the convenience of a bufferless plug and play system. iBlot 2 stack ...
Vor 3 Tagen · Learn the process of probing western blots including blocking the membrane, washing and incubating with primary and secondary antibodies.
- 4 Min.
Vor einem Tag · Western blot analysis was performed at 24, 36, and 48 h post-rESWT (Fig. 2D and E). Results indicated a significant reduction in Bach1 expression at 36 h ( P < 0.001) and 48 h ( P < 0.01) in ...
Vor 2 Tagen · Western blotting is a widely used technique for the detection and analysis of proteins based on their ability to bind to specific antibodies.. It was first described by Towbin, et.al in 1979 and has since become one of the most commonly used methods in life science research.
Vor 2 Tagen · Western blot analysis of apoptosis-related proteins showed that the expressions of Caspase-3, PARP, and Bax were gradually upregulated with the increase of TU concentration (Fig. 1D). Western blot analysis of the expression of endoplasmic reticulum stress-related proteins also revealed that TU promoted the expression of XBP-1, ATF4, and GRP78 in a concentration-dependent manner (Fig. 1 E).
Vor 5 Tagen · Overview of Western Blotting Process Key Steps in Western Blotting. Think of Western blotting as a protein talent show, where the key steps include protein separation, transfer to a membrane, blocking, primary and secondary antibody incubation, and finally, detection. Importance of Protein Detection. Proteins are the Beyoncé of cells – they ...
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