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  1. Translate is a tool which allows the translation of a nucleotide (DNA/RNA) sequence to a protein sequence.

  2. The BLAST report header The top line gives information about the type of program (in this case, BLASTP), the version (2.2.1), and a version release date.

  3. Enter one or more queries in the top text box and one or more subject sequences in the lower text box. Then use the BLAST button at the bottom of the page to align your sequences.

  4. BLAST output can be delivered in a variety of formats. These formats include HTML, plain text, and XML formatting. For NCBI's webpage, the default format for output is HTML.

  5. Enter one or more queries in the top text box and one or more subject sequences in the lower text box. Then use the BLAST button at the bottom of the page to align your sequences.

  6. Enter one or more queries in the top text box and one or more subject sequences in the lower text box. Then use the BLAST button at the bottom of the page to align your sequences.

  7. The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between protein or nucleotide sequences. The program compares nucleotide or protein sequences to sequence in a database and calculates the statistical significance of the matches. This chapter first provides an introduction to BLAST and then describes the practical application of different BLAST programs based on ...

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