Suchergebnisse
Suchergebnisse:
Enter one or more queries in the top text box and one or more subject sequences in the lower text box. Then use the BLAST button at the bottom of the page to align your sequences. To get the CDS annotation in the output, use only the NCBI accession or gi number for either the query or subject. Reformat the results and check 'CDS feature' to ...
28. Nov. 2023 · The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families.
PSI-BLAST allows the user to build a PSSM (position-specific scoring matrix) using the results of the first BlastP run. PHI-BLAST performs the search but limits alignments to those that match a pattern in the query. DELTA-BLAST constructs a PSSM using the results of a Conserved Domain Database search and searches a sequence database.
31. Mai 2010 · In addition to providing BLAST sequence alignment services on the web, NCBI also makes these sequence alignment utilities available for download through FTP. This allows BLAST searches to be performed on local platforms against databases downloaded from NCBI or created locally. These utilities run through DOS-like command windows and accept input through text-based command line switches. There ...
Nucleotide. The Nucleotide database is a collection of sequences from several sources, including GenBank, RefSeq, TPA and PDB. Genome, gene and transcript sequence data provide the foundation for biomedical research and discovery.
Primer-blast tries to find target-specific primers by placing candidate primers on unique template regions that are not similar to other targets. However, in some cases, primer-blast cannot determine if a database sequence is an intended target or not, thus the user guidance might be helpful (For example, when your template is a polymorphic form or a partial region of an entry in the search ...
The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families.
