Suchergebnisse
Suchergebnisse:
An overview of the Hi-C workflow and its applications in research. Figure made using BioRender. Hi-C is a high-throughput genomic and epigenomic technique to capture chromatin conformation. [1] In general, Hi-C is considered as a derivative of a series of chromosome conformation capture technologies, including but not limited to 3C ...
The compatibility of Hi-C with next generation sequencing platforms makes it possible to detect chromatin interactions on an unprecedented scale. This advance gives Hi-C the power to both explore the biophysical properties of chromatin as well as the implications of chromatin structure for the biological functions of the nucleus. A massively ...
- Jon-Matthew Belton, Rachel Patton McCord, Johan Harmen Gibcus, Natalia Naumova, Ye Zhan, Job Dekker
- 2012
A new refinement of the Capture-C method (NG Capture-C) is available. The Hi-C approach extends 3C-Seq to map chromatin contacts genome-wide, and it has also been applied to studying in situ chromatin interactions. In this method, DNA-protein complexes are crosslinked with formaldehyde.
The aim of this paper is to provide guidelines for analyzing and interpreting data obtained with genome-wide 3C methods such as Hi-C and 3C-seq that rely on deep sequencing to detect and quantify pairwise chromatin interactions genome-wide.
- Bryan R. Lajoie, Job Dekker, Noam Kaplan
- 10.1016/j.ymeth.2014.10.031
- 2015
- 2015/01
Hi-C uses high-throughput sequencing to find the nucleotide sequence of fragments and uses paired end sequencing, which retrieves a short sequence from each end of each ligated fragment. As such, for a given ligated fragment, the two sequences obtained should represent two different restriction fragments that were ligated together in ...
Hi-C is a chromosome conformation capture (3C)-based technology to detect pair-wise chromatin interactions genome-wide, and has become a benchmark tool to study genome organization. In Hi-C, chromatin conformation is first captured by chemical cross-linking of cells.
The compatibility of Hi-C with next generation sequencing platforms makes it possible to detect chromatin interactions on an unprecedented scale. This advance gives Hi-C the power to both explore the chromatin biophysics as well as the implications of chromatin structure in the biological functions of the nucleus.
